PHYSICS SEMINAR
PROTEIN STRUCTURE AND LIGAND-RECEPTOR BINDING POTENTIAL LANDSCAPE REVEALED WITH AFM IN SOLUTION
Jie Yang
Department of Physics
University of Vermont
Friday, September 16, 2011
1:30 p.m.
Nicolls 315
Atomic Force Microscopy (AFM) has shown its potential to reveal the molecular structure of proteins in solution under physiological conditions. AFM can also serve as the molecular force probe to study inter-molecular bonds at the single bond level. In this talk I will show how the binding potential landscape of a protein, the cholera toxin B-oligomer, can be obtained with AFM as the molecular force probe. I will also show the binding character revealed with AFM on how DNA was tethered to a substrate. I will also show high-resolution images of biological macromolecules in solution obtained with AFM.
