Research - Dr. Elizabeth Prusak
Dr. Elizabeth Prusak
Department of Biochemistry
Dr. Prusak is currently working on two research projects. The first involves the study of metabolic pathways of acetone degradation and has the potential to help eliminate environmental contaminants. Her second project, in collaboration with her colleague Dr. Mihai Scarlete, researches the application of miniature optical sensors called silicon oxynitride waveguides to monitor biochemical reactions, and could lead to development of a new generation of small biosensors.
1. Metabolic Pathways of Acetone Degradation
Dr. Prusak aims to identify soil bacteria able to degrade acetone and to characterize their metabolic pathways. Very few microorganisms are known to use acetone as a carbon source, and we don't clearly understand how bacteria metabolize acetone. Yet biodegradation of acetone is very important because although acetone is volatile and dissipates into the environment instead of accumulating in a given area, it still remains toxic. Understanding of acetone degradation could contribute to elimination of important environmental contaminants. Characterization of the acetone-utilizing enzyme is also important to take the full advantage of the metabolic process.
2. Application of Silicon Oxynitride Waveguides
Dr. Prusak and Dr. Mihai Scarlete are collaborating to apply Dr. Scarlete's waveguide microsystems to monitor biochemical reactions. Many organic compounds absorb light only in the ultra-violet region, and traditional waveguides do not perform well below 400 nanometres. Dr. Scarlete's use of silicon oxynitrides, on the other hand, allows for the creation of a transparent waveguide applicable at a molecular level, and which therefore has potential applications in biochemical analysis. Polymer-Assisted Chemical Vapour Deposition is used to produce the waveguides and they are tested to determine their use in monitoring enzyme-catalyzed reactions or binding of biochemical molecules.
